Larval Zebrafish Brain Anatomy

Due to their small size and transparency zebrafish larvae are amenable to a range of fluorescence microscopy techniques. In a recent paper owen randlett florian engert alex schier and colleagues have created new methods to analyze the anatomy of the larval zebrafish brain.

Multiscale Atlas Of The Larval Zebrafish Brain Zuse Institute

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With the development of sensitive genetically encoded calcium indicators this has extended to the whole brain imaging of neural activity with cellular resolution.

Larval zebrafish brain anatomy. Currently our ability to record activity from zebrafish neurons vastly outweighs our anatomical knowledge of this brain. Normal table of postembryonic zebrafish development. Brain browser a tool for 3d visualization of transgene reporter patterns in 6 dpf larval zebrafish burgess lab.

Here we present ssem data for the complete brain of a larval zebrafish danio rerio at 55 days post fertilization. These data are used to generate the first brain wide wiring diagram with single cell resolution for a vertebrate species. This material is from the 4th edition of the zebrafish book.

Furthermore detailed anatomical ontologies are curated zfin anatomical ontologies but their application to larval stages is handicapped by the fact that the ontologies rely on morphologically identifiable structures which are scarce in the larval zebrafish brain. Nonretinal projections to the optic tectum of larval zebrafish. Staging by externally visible anatomy of the living fish parichy elizondo mills gordon engeszer.

The larval zebrafish is increasingly being used to address fundamental questions of nervous system organization and circuit function at the whole brain level vanwalleghem et al 2018 yet its neuro anatomy is still relatively uncharted. Although experimental analysis of the embryo and larva is extensive and the morphology has been well documented descriptions of adult zebrafish anatomy and studies of development of the adult structures and organs together with techniques for working with adults are lacking. These efforts were recently transformed by advances in computing sample handling and imaging techniques but high resolution examination of entire brains remains a challenge.

The 5th edition is available in print and within the zfin protocol wiki. Here we introduce a novel resource which will be beneficial to approaches that attempt to tie neuronal. Generated a digital atlas of the larval zebrafish brain containing over 2000 neuron morphologies most of them previously unknown.

Therefore the larval brain is somewhat under annotated which will. Here we demonstrate that neurons within the rh project to the tectum in larval zebrafish. The tecta of various teleost species including zebrafish are known to receive input from the retina and from a host of regions throughout the brain.

Swimming Zebrafish